Investigating inflammatory processes of plaque destabilization using proteomics
SSAR 2022
Lasse Lorentzen
N-terminomics investigation of protelytic ECM remodelling
Proteomics workflow
Differentially regulated free N-termini

Most regulated free N-termini are non-canonical

Degradation of Collagen type VI

Degradation of other Collagens

Degradation of Fibronectin

Degradation of other basement membrane proteins

Sequence motif analysis

Summary
- Soft plaques are associated with lower level of ECM proteins
- Soft plaques are associated with increased abundance of proteolytic enzymes
- ECM proteins are subject to proteolytic degradation
- ECM degradation is consistent with MMP activity
Thanks
- Prof. Michael Davies
- Prof. Henrik Sillesen, Prof. Jonas Eiberg, Karin Yeung
- Christine Chuang
- Protein Oxidation Group
Extensive coverage of the proteome and matrisome

Over-degradation of inhibitors

Activation of complement system

Activation of complement system
